An 'antigen capture'ELISA for secretory immunoglobulin A antibodies to hepatitis B surface antigen in human saliva
MA Siddiqi, S Abdullah - Journal of immunological methods, 1988 - Elsevier
An enzyme-linked immunosorbent assay (ELISA) was developed which permits the assay of
specific secretory immunoglobulin A (sIgA) antibodies against hepatitis B surface antigen
(HBsAg) in saliva. The assay is based on the binding of sIgA antibodies present in saliva to
microtitre plates coated with excess of F (ab′) 2 anti-secretory component antibodies,
followed by the addition of specific antigen, HBsAg and finally peroxidase-labelled anti-
HBsAg. The assay is fast, simple, reproducible and antigen specific as shown by total …
specific secretory immunoglobulin A (sIgA) antibodies against hepatitis B surface antigen
(HBsAg) in saliva. The assay is based on the binding of sIgA antibodies present in saliva to
microtitre plates coated with excess of F (ab′) 2 anti-secretory component antibodies,
followed by the addition of specific antigen, HBsAg and finally peroxidase-labelled anti-
HBsAg. The assay is fast, simple, reproducible and antigen specific as shown by total …
